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KMID : 0903519930360030139
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Journal of the Korean Society of Agricultural Chemistry and Biotechnology
1993 Volume.36 No. 3 p.139 ~ p.145
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Varietal Classification of barley by lsozymes and Restriction fragment length polymorphisms(RELPs)
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은무영/Eun, Moo Young
박노동/진병순/이은섭/Park, Ro Dong/Jin, Byung Soon/Lee, Eun Seop
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Abstract
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The methods of isoelectric focusing of 4 isozymes in polyacrylamide horizontal slab gels and restriction fragment length polymorphisms (RFLPs) were applied to characterize the biochemical phenotypes of 19 cultivars of barley. Among 19 barley cultivars screened, 7 esterase, 3 phosphoglucose isomerase, 4 peroxidase and 2 alcohol dehydrogenase isozyme phenotypes were distinguished by isoelectric focusing. When purified DNA of each cultivar was digested with restriction enzyme EcoRV and analyzed its RFLPs with barley DNA markers pMSU 51 or pMSU 71, two distinct RFLP patterns were shown. Based on the four isozymes and two RELP polymorphisms. 19 cultivars of barley were classified into 13 biochemical phenotypes. Phylogenetical relationships among 13 biochemical phenotypes classified were determined using Nei´s F-statistics and the phylogenetic dendrogram was constructed.
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